Thus, in conjunction with our published data reporting no change in DMPK and SIX5 mRNA expression levels in DM1 unmanipulated as compared to gene-edited myoblasts (see ref. 25), this strongly suggests that local gene expression is unaffected by abnormal methylation at the DM1 locus, at least not in embryonic stem cells and patient myoblasts. This evidence concerns the gene SIX5 and myotonic dystrophy type 1.