CD8A and neoplasm: To further characterize the performance of UMIS in our discovery cohort of 100 UM metastases, we benchmarked its ability to predict co-culture anti-tumor reactivity against several other tumor biopsy metrics including TMB, percentage of infiltrating CD8+ T cells, and several published gene expression profiles for T cell inflammation (Fig. 5g, h and Supplementary Data 16)30,39–41.