Through Spearman correlation analysis, the correlation analysis between SCD and immune checkpoints found that SCD was negatively correlated with all immune checkpoints, and significantly negatively correlated with CD274, LAG3, CD47, CD40, TNFSF14, TGFB1, and TNFSF13 (P<0.05) (Fig 7C). The gene discussed is CD274; the disease is Schnyder corneal dystrophy.