Note that other human PCa cell lines, such as DU145, 22Rv1, and LAPC-4 (see Supplemental Figure 5H and ref. 7), that do not express ERα did not show any significant modulation by either E2 or propyl pyrazole triol (PPT, a specific agonist of ERα), irrespective of their AR status. This evidence concerns the gene ESR1 and posterior cortical atrophy.