ACTA2 and renal fibrosis: Thus, subsequent experiments used Cf48 LNA1 plus LNA2 (referred to hereafter as Cf48 LNA), which produced a substantial reduction in renal fibrosis as shown by Western blotting for collagen I and Acta2 (Figure 6, B and C), and collagen deposition based on Masson’s trichrome staining (Figure 6, D and E).