A significant reduction in renal fibrosis in KO versus WT mice on day 28 of FAN was evident based on Masson’s trichrome staining, Western blotting for collagen I and Acta2, and immunofluorescent staining for collagen IV, Acta2, and fibronectin (Figure 5, F–I, and Supplemental Figure 13, B–E). This evidence concerns the gene ACTA2 and renal fibrosis.