In our study, ACE treatment activated the AMPK-mTOR signaling pathway, resulting in the inhibition of mTOR expression and an upregulation of downstream autophagy-related mRNA expression (ULK, Atg5, Atg7).Although autophagy and leptin resistance have been associated with obesity, the relationship between autophagy and leptin remains unclear. Here, ATG7 is linked to obesity due to melanocortin 4 receptor deficiency.