Our results indicated that LCSLCs derived from the A549 and H460 cells exhibited stronger self-renewal and tumor growth abilities, compared with the A549 and H460 cells.Furthermore, H460 cell-derived spheroids display greater tumorigenicity in vivo, likely attributable to high levels of DNMT1, low levels of miR-152-3p, miR-152-3p promoter hypermethylation, and SOS1 overexpression. The gene discussed is SOS1; the disease is neoplasm.