Astrocytes also clustered into two distinct patterns, with Astrocyte_A cells showing lower expression of GFAP and localizing to distant brain areas outside of the tumor, while Astrocyte_B cells showed higher expression of GFAP, and localized to the border regions of both PDGfp and BrM tumors (Supplementary Figs. 5h and 6d). This evidence concerns the gene GFAP and neoplasm.