MUC5AC and cystic fibrosis: The isolates nmFLRO1, mFLRO1, SH1B, SH2D, and SH3A and laboratory strains PAO1 and PA14 were cultured in synthetic cystic fibrosis medium 2 (SCFM2), a chemically defined medium complemented with mucin and DNA developed to mimic the nutritional environment of the CF lung (42, –, 44).