Protein expression observed by high-dimensional multiplex imaging of a human patient tumor among pan-cytokeratin (pan-CK+) cancer cells and standard immunofluorescence and quantification by area for CD68 and CD8A on a ccRCC tissue microarray (TMA) staining also indicated diffuse infiltration of CD4+, CD8A+, and CD68+ cells in situ (Figure 1D and Supplemental Figure 1, A and B; supplemental material available online with this article; https://doi.org/10.1172/JCI173934DS1). This evidence concerns the gene CD8A and nonpapillary renal cell carcinoma.