In order to examine the correlation between VHL and PIK3R3, we collected cell lysates from a panel of renal epithelial cell lines (such as 293T and HKC), VHL-positive RCC cell lines (such as Caki-1 and ACHN) and VHL-null RCC lines (including UMRC2, UMRC6, 786O, A498, RCC4, and Caki-2). Here, PIK3R3 is linked to renal cell carcinoma.