In the related cells of hepatocellular carcinoma, the correlation between ARF5 expression and immune cells is significantly greater than that of hepatocytes, and the signaling pathway that may be regulated by ARF5 co-expression gene network is significantly related to nonalcoholic fatty liver, indicating that ARF5 may play an important role in the progression of nonalcoholic fatty liver to hepatocellular carcinoma, and may participate in the regulation and destruction of the immune microenvironment of liver tissue. Here, ARF5 is linked to non-alcoholic fatty liver.