Upon co-culturing HL-60N cells transfected with si-TOB1 or si-NC, activated by TTCS stimulation, with AGS and HGC-27 cells for 48 hours, it was observed that the gastric cancer cells co-cultured with HL-60N cells from the si-TOB1 group exhibited enhanced proliferative (Figure 7H, P = 0.014 and P = 0.016), migratory (Figure 7I, P=0.004 and P=0.036), and invasive (Figure 7J, P =0.023 and P=0.045) capabilities. This evidence concerns the gene TOB1 and gastric cancer.