By combining insights from website predictions and previous research, we opted for CD8, CD4, FOXP3, CD20, CD68, and CD66b as markers to characterize cytotoxic T cells, Th cells, Tregs, B cells, macrophages, and neutrophils, respectively, to investigate the role of TOB1 in the context of the gastric cancer microenvironment. Here, CD4 is linked to gastric cancer.