ACTA1 and triple-A syndrome: Consistently, immunofluorescent double staining demonstrated that colchicine treatment reduced the tubulin levels in α-SMA-positive SMCs from AAA lesions from both CaPO4-injured and Ang-II-infused mice (Fig. 2E, Fig. S5D), but colchicine did not affect tubulin expression in Mac2-positive macrophages from these lesions (Fig. 2F, Fig. S5E).