ACTA1 and triple-A syndrome: Immunofluorescent double staining showed that the expression of SMC contractile and early differentiation marker TAGLN in α-SMA-positive SMCs increased significantly in AAA lesions from colchicine-treated mice (Fig. 3A), whereas KLF4+α-SMA+ and CD68+α-SMA+ double-positive SMCs were decreased in AAA lesions from these mice (Fig. 3B-C).