Meanwhile, the expressions of phosphorylated Drp1 (p-Drp1, S616) and Fis1 were decreased, while OPA1, MFN1 and MFN2 were increased in HCT116 and RKO cells with PTPLAD1 overexpression, and a converse effect was found in CRC cells transfected with two siRNAs against PTPLAD1 (Fig. 3C), suggesting that PTPLAD1 negatively regulates mitochondrial fission in CRC. Here, FIS1 is linked to colorectal carcinoma.