To determine the role of PTPLAD1 in several CRC lines with different invasive potentials, we performed western blot assay in SW480 and SW620 (two cell lines with different invasion abilities obtained from one patient), HCT116 and HCT116-i8 (HCT116 highly invasive cell screened by using 8 rounds of invasion assay) (Supplementary Fig. 1A), as well as FHs 74Int (normal small intestine epithelial cell). Here, HACD3 is linked to colorectal carcinoma.