Next, we performed IHC assay to detect the level of PTPLAD1 in 94 cases of CRC tissues with survival and TNM data, and found that the expression level of PTPLAD1 did not significantly change between normal tissues and early stage of CRC tissues (stages 1 and 2), but it was gradually decreased in late stage of CRC tissues (stages 3 and 4). This evidence concerns the gene HACD3 and colorectal carcinoma.