To confirm the role of p-Y259 of PHB on CRC metastasis, firstly, endogenous PHB-deficient cells were established by using CRISPR/Cas9 technology (Supplementary Fig. 4B), phosphorylation-mimetic Y259D mutant and dephosphorylation-mimetic Y259A mutant were then re-introduced into PHB-knockout (KO) cells, respectively. Here, PHB1 is linked to colorectal carcinoma.