It was worth noting that although the primary tumor volumes in the R@MMSNs-P group were a little larger than those in the G@MMSNs-P group, the pulmonary metastatic nodules in the R@MMSNs-P group were obviously less than those in the G@MMSNs-P group, which was attributed to the effective downregulation of Twist protein by using siRNA-loaded MMSNs. The gene discussed is TWIST1; the disease is neoplasm.