The correlation analysis between FABP1 expression levels and the clinicopathological features of GBC patients demonstrated that FABP1 expression level in DI region was significantly correlated with lymph node metastasis (P=0.028), but not correlated with other clinical factors such as patient age, gender, tumor size, degree of differentiation and nerve invasion (P>0.05) (Supplementary Table S3). The gene discussed is FABP1; the disease is metastatic malignant neoplasm in the lymph nodes.