Nevertheless, Kashofer K et al. [33] detected the hotspot mutation of PIK3CA at E545K and E453K in the early stage of CESC with a microinvasive pT1 stage and reported that the mutation rates of PIK3CA E545K and E453K were 5/8 (62%) in CESC with pT1a, 7/15 (70%) in CESC with pT1b and 10/15 (66%) in CESC with pT2/3. This evidence concerns the gene PIK3CA and cervical squamous cell carcinoma.