However, when cotreated with IAM or AOAA and PAG to remove exogenous or endogenous H2S, the inhibitory effects of GO on the activation of NF-κB, NLRP3 inflammasome complex, and pyroptosis in LPS-induced ALI were significantly weakened (Figure 6I, 6J). This evidence concerns the gene NFKB1 and acute respiratory distress syndrome.