Groups: (1) control; (2) hypothyroidism group; (3) hypothyroidism + mustard oil (1 mL/kg b.w.) group; (4) hypothyroidism + mustard oil (2 mL/kg b.w.); (5) hyperthyroidism group; (6) hyperthyroidism + mustard oil (1 mL/kg b.w.); (7) hyperthyroidism + mustard oil (2 mL/kg b.w.)Procedures: measurement of level of thyroid hormones, liver and kidneys parameters and assessment Time: 8 weeks. This evidence concerns the gene TG and hypothyroidism.