In order to explore the impact of GPC4 on the progression of these cancer types, the expression of GPC4 was transiently disrupted by a GPC4-targeted CRISPR double nickase consisting of GPC4-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library (CRISPR/Cas9 GPC4), and the effects on proliferation of different cancer cell lines isolated from cancer patients was investigated. This evidence concerns the gene GPC4 and cancer.