In order to elucidate the effect of overexpression of GPC4 on the proliferation of glioblastoma and non-small cell lung carcinoma cells, SNB-75, SF-295 and HOP-92 cells were either transfected with pCMV3-C-GFPSpark GPC4 overexpression vector (overexpression GPC4) or pCMV3-C-GFPSpark negative control vector (overexpression control) or were left untreated to monitor their proliferation rate in the absence of any treatment. The gene discussed is GPC4; the disease is glioblastoma.