Also, experimental in vitro investigations knocking out GPC4 expression with CRISPR/Cas9 in cell lines originating from cancer patients revealed the divergent effects of GPC4 in glioblastoma and non-small cell lung carcinoma, i.e., attenuation of proliferation in glioblastoma and promotion of proliferation in non-small cells lung adenocarcinoma cells. Here, GPC4 is linked to cancer.