HIF1A and acute kidney injury: By observing the results of the immunohistochemical HIF-1 expression in the AKI + HBO group (Figure 7C) and comparing them with those of the AKI group, it was not clear if HBO preconditioning affected HIF-1 tissue expression, and in order to evaluate those results, we also performed a Western blot analysis to quantify the HIF-1α expression.