Complementary exams such as ECG and echocardiographic examination are important and have been recommended to screen for cardiac disease in T. cruzi-infected patients [22,29,78,87,106,107,108], and serum cardiac troponin I (cTnI) has been increasingly studied as an early biomarker of parasitic myocarditis in dogs with CD [81,82,113]. Here, TNNI3 is linked to heart disorder.