EV-MAP technology has been used to isolate tumor-specific biomarkers and T cell EVs associated with acute ischemic stroke from plasma or serum [25] By loading tEVs instead of plasma onto the EV-MAP microfluidic chips, co-isolated contaminants, such as albumin and lipoproteins, which are abundant in the plasma, are significantly reduced (Appendix A). This evidence concerns the gene ALB and neoplasm.