Additional studies support the existence of ANCA/autoantigens in MPO/PR3-ANCA-negative vasculitides including identification of elastase-ANCA in adult ANCA-negative glomerulonephritis [29] and the presence of alpha-enolase, a potential cytosolic autoantigen, in 82% of adults with predominantly ANCA-negative EGPA [30]. This evidence concerns the gene MPO and eosinophilic granulomatosis with polyangiitis.