It was assumed that the DEG profiles resulted in the clinically meaningful expression of certain genes, as the qRT-PCR of CCNE1 and SERPINE1 after PFD treatment and ACOT7, CCNB2, CDK1, and SERPINE1 after NTD treatment showed a similar trend, although not significant, to the transcriptome signature. The gene discussed is ACOT7; the disease is neural tube defect.