Interestingly, in colorectal cancer (CRC), CYT has been shown associate with different mutational events and -CYThigh tumors are related to an increased tumor mutational burden (TMB) [24,25,26] and upregulate the expression levels of immune checkpoints, including PD-1, PD-L1/2, CTLA-4, LAG3, and IDO1 [22,24]. Here, CTLA4 is linked to colorectal carcinoma.