Strikingly, neither infections of OKs cultures with monocultures of P. gingivalis and F. nucleatum or the co-culture nor co-infection with both bacteria previously grown individually affected the total TLR4 protein levels under the evaluated experimental conditions, which suggests that neither P. gingivalis nor F. nucleatum alone increases TLR4 levels upon infection (Figure 7). This evidence concerns the gene TLR4 and coinfection.