By validating the expression profiles of six specific genes (MIR205HG, KRT5, KRT6A, KRT6C, SERPINB5, and DSG3), selected based on a previously established 53-gene signature, we consistently observed higher expression levels in squamous cell carcinoma (SCC) compared to adenocarcinoma (ADC). The gene discussed is SERPINB5; the disease is adenocarcinoma.