BMDMs were differentiated from C57/BL6 mice with M-CSF for 6 days, as described in Section 4, and then cultured with conditioned media collected from the 4T1 mouse mammary carcinoma cells and diluted at a 1:1 ratio with complete DMEM medium [32,33] for 24 h. The gene discussed is CSF1; the disease is breast carcinoma.