Through Western blot analysis, MTT assay, and other research methods, it was found that β-caryophyllene oxide (68) not only inhibited the constitutive activation of PI3K/AKT/mTOR/S6K1 signaling cascade in human prostate cancer PC-3 and breast cancer MCF-7 cells; it also causes the activation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 MAPK in tumor cells, and down-regulates various gene products related to cell proliferation, anti-apoptosis, and metastasis. Here, AKT1 is linked to neoplasm.