It is reported that IgM anti-HBc was significantly higher in AHB, while HBV DNA level was significantly higher in acute exacerbation of chronic hepatitis B (CHB-AE), and IgM-HBc level of ≥8 S/CO (sample to the cut-off value) and an HBVDNA level of <5.5 logIU/mL are considered useful for the differentiation of AHB and CHB-AE (sensitivity 98.1%, specificity 86.2%) [7]. This evidence concerns the gene CD40LG and chronic hepatitis B virus infection.