Pathway analysis showed that the immune-high subtype was characterized by inhibition (negative Z-score) of six pathways associated with cytokine production and cell-mediated immune response (neuroinflammation signaling pathway, PD-1, PD-L1 cancer immunotherapy pathway, TREM1 signaling, pathogen-induced cytokine storm signaling pathway, role of pattern recognition receptors in recognition of bacteria and viruses, and Th1 pathway) (Figure 10E and Table S5). This evidence concerns the gene CD274 and cancer.