GPBAR1 and cancer: Supplemental Figures S3 and S4 demonstrates that in human MDA-MB-231 and SUM159 cell lines, TGR5 agonism failed to demonstrate anti-cancer efficacy on cell proliferation, viability, or migration (Figure S4A–F). Similarly, in murine E0771 cells, INT-777 failed to blunt progression or viability, with a negligible impact on migration (Figure S3G–I).