In the univariate analysis, significant independent predictors of worsening CFS were clinical T stage ≥ 3 (hazard ratio (HR) 10.28, 95% confidence interval (CI) 1.45–73.04, p = 0.020), biopsy and surgical GGG = 5 (HR 20.98, 95% CI 2.32–189.76, p = 0.007, and HR 27.49, 95% CI 2.91–260.00, p = 0.004), PSA at treatment start ≥ 0.5 ng/mL (HR 9.27, 95% CI 1.53–56.05, p = 0.015). This evidence concerns the gene KLK3 and myalgic encephalomeyelitis/chronic fatigue syndrome.