Our findings align with the previous research indicating elevated HIF-1α expression across various cancer types, including renal cancer [27,28], glioblastoma [29], non-small-cell lung cancer [30], thyroid carcinoma [31], bladder cancer [32], cervical cancer [33], endometrial carcinoma [34], gastric cancer [35], and rectal cancer [36,37]. The gene discussed is HIF1A; the disease is rectal cancer.