Western immunoblotting analysis of human skeletal muscle showed equal migrated bands at ~130 kDa with similar intensity in all samples both before and after 8 weeks of mineralocorticoid blockade, indicating no difference in the expression of eNOS between the individuals with type 2 diabetes and the control group, or in the individual groups before or after MR blockade (Figure 5c,d; Figure S3). This evidence concerns the gene NR3C2 and type 2 diabetes mellitus.