The quantitative assessment of the CD8 + type T cell predominance in tumor tissues was also performed, and the results showed that the HPNDs@EcN + US group exhibited good pro-CD8 + type T cell infiltration in tumor localization, further illustrating the ability of HPNDs@EcN + US to induce tumor immune responses in the body (Fig. 6E and F). The gene discussed is CD8A; the disease is neoplasm.