Furthermore, our investigations indicate that PREX2 serves as a pivotal regulator of radiation-induced immunogenic cell death (ICD), inhibiting exposure of calreticulin (CRT) on the cell surface, suppressing HMGB1 and ATP release, and impeding interferon-I (IFN-I) production in CRC cells exposed to 4 Gy radiation. Here, HMGB1 is linked to colorectal carcinoma.