The blood chemistry data analysis showed that compared to IBS/FD patients the NCWS patients (overall and both NCWS FCP + and NCWS FCP-) had a higher frequency of elevated TSH (P = 0.0018, P = 0.037 and P = 0.016, respectively) and of ANA positivity (P = 0.0001 for all) (see Online Source 2). This evidence concerns the gene BTG3 and irritable bowel syndrome.