To uncover the mechanisms underlying the improvement in hepatic steatosis by a KD, we performed RNA-Seq analysis on mouse livers and found that the mRNA expression profile of Fgf21 signaling was significantly upregulated, especially Fgf21, Klb, and Fgfr1 (Fig. 3A). This evidence concerns the gene FGFR1 and Hepatic steatosis.