While not directly comparable to cells in the lung, flow cytometric analysis of B16.F10 cells grown for 11 days in vivo nonetheless showed expression of SEMA3A within the TME, mainly from tumor cells but also blood endothelial cells (BEC), lymphatic endothelial cells (LEC) and some CD45+ cells (Supplementary Fig. 3D, E). This evidence concerns the gene SEMA3A and neoplasm.