At 36 hours post-infection, we found that the yield of PRRSV-2-GFP progeny virus was negatively regulated by 13 RABs (RAB3D, RAB6B, RAB9B, RAB11A, RAB22A, RAB22B, RAB24, RAB27A, RAB34, RAB39A, RAB40B, RAB43, and RAB44) and positively regulated by 29 RABs (RAB2B, RAB3A, RAB3B, RAB4A, RAB4B, RAB5A, RAB5B, RAB5C, RAB6A, RAB7A, RAB8A, RAB8B, RAB10, RAB11B, RAB13, RAB14, RAB15, RAB18, RAB26, RAB28, RAB35, RAB36, RAB38, RAB39B, RAB40A, RAB42, RAB45, RABL2B, and RABL3) (Fig 1B). Here, RAB40B is linked to infection.