PSEN1 and Alzheimer disease: 5xFAD consists of transgenic mice overexpressing mutant human amyloid-β precursor protein 695 (APP) with the Swedish (K670N, M671L), Florida (I716V), and London (V717I) Familial AD (FAD) mutation along with human presenilin 1 with two FAD mutations (M146L and L286V).