Considering the accepted role of MTs in innate immunity and infection [92,109,110,111,112], their essential roles in intracellular trafficking and cell morphology (reviewed in [113]), the involvement of the ZIKV NS5 protein in virus replication and evasion from the interferon (IFN)-associated immune response, and its association with the centrosome or microtubule (MT) organized centre (MTOC) during mitosis and cell division [95,114,115,116], we aimed to study the effect of the ZIKV NS5 protein on MTs to ascertain potential interplay at this level. This evidence concerns the gene RAF1 and infection.