Astragalus polysaccharides can reduce serum RANKL, osteocalcin, and TNF-α levels in OVX-induced osteoporosis mice and increase the content of osteoprotegerin (OPG) to prevent osteoporosis (Huo and Sun, 2016; Ou et al., 2019). The gene discussed is TNFSF11; the disease is osteoporosis.