The nNGM (national Network Genomic Medicine lung cancer) demands the detection of defined areas including variant hot spots in 26 Genes (ALK, BRAF, CTNNB1, EGFR, ERBB2, FGFR1, FGFR2, FGFR3, FGFR4, HRAS, IDH1, IDH2, KEAP1, KRAS, MAP2K1, MET, NRAS, NTRK1, NTRK2, NTRK3, PIK3CA, PTEN, RET, ROS1, STK11, TP53). The gene discussed is PTEN; the disease is lung cancer.