To understand the influence of PDPK1 on the transcriptome in N-MYC amplified neuroblastoma cells, we engineered CHP-134 cells so that endogenous PDPK1 is expressed in-frame with a FKBP12(F36V)-2xHA tag, allowing PDPK1 to be degraded by the dTAG method [17]. The gene discussed is MYCN; the disease is neuroblastoma.