Figure 1C shows a fluorescence image of a CT26 tumor that was labeled with the custom 4-plex panel, demonstrating robust membrane localization for CD3ε, CD4 and CD8α biomarkers and nuclear localization for FoxP3, consistent with the subcellular localization pattern that was observed in brightfield IHC images (Fig. 1B). Here, FOXP3 is linked to neoplasm.