PRPF8 and retinitis pigmentosa 1: To reveal the role and importance of the fine-tuning of hBrr2 by PRPF8 in the human spliceosome and the consequences of its disruption by RP mutations, we herein generated patient iPSCs-derived retinal organoids (ROs), and retinal pigment epithelium (RPE) carrying the pathogenic PRPF8 RP type 13 c.6926 A > C (p.H2309P) heterozygous missense mutation and their corrected isogenic controls (using the CRISPR/Cas9 genome editing).